Rat glutamic acid decarboxylase, GAD ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Glutamate decarboxylase 1,67 kDa glutamic acid decarboxylase(GAD-67),Glutamate decarboxylase 67 kDa isoform, GAD1, CPSQ1;
GAD; SCP; GAD-67; glutamate decarboxylase 1;

Search name
Rat Glutamate decarboxylase 1 ELISA Kit, Rat glutamic acid decarboxylase ELISA Kit, Rat GAD ELISA Kit, Rat GAD-67 ELISA Kit, Rat Glutamate
decarboxylase 67 kDa isoform ELISA Kit, Rat GAD1 ELISA Kit, Rat CPSQ1 ELISA Kit, Rat SCP ELISA Kit, Rat glutamate decarboxylase 1 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat glutamate decarboxylase 1,Glutamate decarboxylase 1
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate decarboxylase 1. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Glutamate decarboxylase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Glutamate decarboxylase 1, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Glutamate decarboxylase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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