Rat Glutathione peroxidase 1,GPX1 ELISA Kit

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  • Model: ELA-E0295r
  • 20 Units in Stock
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Rat Glutathione peroxidase 1, GPX1 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Glutathione peroxidase 1(GPx-1,GSHPx-1),Cellular glutathione peroxidase, Gpx1,Gpx; GPx-1; AI195024; AL033363; CGPx;

Search name
Rat Glutathione peroxidase 1 ELISA Kit, Rat GPx-1 ELISA Kit, Rat GSHPx-1 ELISA Kit, Rat GPx1 ELISA Kit, Rat Cellular
glutathione peroxidase ELISA Kit, Rat GPx ELISA Kit, Rat AI195024 ELISA Kit, Rat AL033363 ELISA Kit, Rat CGPx ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat glutathione peroxidase 1,GPx-1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPx-1. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GPx-1 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain GPx-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GPx-1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

PDF manual download

 

Reference

Article: Changes in nitric oxide, carbon monoxide, hydrogen sulfide and male reproductive hormones in response to chronic restraint stress in rats
Author:  AmiraMoustafa
Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt
Received 30 July 2020, Revised 1 September 2020, Accepted 22 October 2020, Available online 29 October 2020.
Free Radical Biology and Medicine
Available online 29 October 2020

DOI:doi.org/10.1016/j.freeradbiomed.2020.10.315

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