Rat Glycosylated hemoglobin A1c, HbA1c ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HbA1c,Glycosylated hemoglobin A1c, hemoglobin A1c, HbA1c, A1C, Hb1c;
Search name
Rat HbA1c ELISA KIT ,Rat Glycosylated hemoglobin A1c ELISA KIT ,Rat hemoglobin A1c ELISA KIT ,Rat A1C ELISA KIT ,Rat Hb1c ELISA KIT ,
Intended use
Rat HbA1c ELISA KIT allows for the in vitro quantitative determination of rat Glycated hemoglobin A1c concentrations in serum, Plasma, Urine,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat HbA1c ELISA KIT has been pre-coated with an antibody specific to Glycated hemoglobin A1c. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glycated hemoglobin A1c and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycated hemoglobin A1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycated hemoglobin A1c in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 3.12-200ng/ml
Rat HbA1c ELISA KIT PDF manual download
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