Rat Growth Hormone Releasing Peptide- 2, GHRP- 2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GHRP-2,Protein M46,Motilin-related peptide,Growth hormone secretagogue,Growth hormone-releasing peptide, ghrelin,MTLRP, appetite-regulating hormone, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin preprohormone, growth hormone secretagogue, growth hormone-releasing peptide, motilin-related peptide, prepro-appetite regulatory hormone, ghrelin/obestatin prepropeptide
Search name
Rat GHRP-2 ELISA KIT ,Rat Protein M46 ELISA KIT ,Rat Motilin-related peptide ELISA KIT ,Rat Growth hormone secretagogue ELISA KIT ,Rat Growth hormone-releasing peptide ELISA KIT ,Rat ghrelin ELISA KIT ,Rat MTLRP ELISA KIT ,Rat appetite-regulating hormone ELISA KIT ,Rat ghrelin ELISA KIT ,Rat growth hormone secretagogue receptor ligand ELISA KIT ,Rat ghrelin/obestatin preprohormone ELISA KIT ,Rat growth hormone secretagogue ELISA KIT ,Rat growth hormone-releasing peptide ELISA KIT ,Rat motilin-related peptide ELISA KIT ,Rat prepro-appetite regulatory hormone ELISA KIT ,Rat ghrelin/obestatin prepropeptide ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat GLRP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GLRP2, During the reaction, GLRP2 in the sample or standard competes with a fixed amount of biotin-labeled GLRP2 for sites on a pre-coated Monoclonal antibody specific to GLRP2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GLRP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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