Rat HCN4 ELISA Kit

Starting at: $756.00

  • Model: ELI-14472r
  • 20 Units in Stock
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Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, HCN4 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4,HCN4,SSS2,hyperpolarization activated cyclic nucleotide-gated cation channel 4, Bcng3,Brain cyclic nucleotide-gated channel 3,BCNG-3,HAC4,HAC-4

 

Search name

Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 ELISA KIT ,Rat HCN4 ELISA KIT ,Rat SSS2 ELISA KIT ,Rat hyperpolarization activated cyclic nucleotide-gated cation channel 4 ELISA KIT ,Rat  Bcng3 ELISA KIT ,Rat Brain cyclic nucleotide-gated channel 3 ELISA KIT ,Rat BCNG-3 ELISA KIT ,Rat HAC4 ELISA KIT ,Rat HAC-4 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4,HCN4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HCN4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HCN4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HCN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HCN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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