Rat Heat shock protein beta- 1, HSPB1 ELISA KIT
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Heat shock protein beta-1(HspB1),Heat shock 27 kDa protein(HSP 27),; HSP 27; CMT2F; HMN2B; HS.76067; HSP28; Hsp25; SRP27;
28 kDa heat shock protein; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock protein
beta-1; stress-responsive protein 27; heat shock 27kDa protein 1,

Search name
Rat Heat shock protein beta-1 ELISA Kit, Rat HspB1 ELISA Kit, Rat Heat shock 27 kDa protein ELISA Kit, Rat HSP
27 ELISA Kit, Rat CMT2F ELISA Kit, Rat HMN2B ELISA Kit, Rat HS.76067 ELISA Kit, Rat HSP28 ELISA Kit, Rat
Hsp25 ELISA Kit, Rat SRP27 ELISA Kit, Rat stress-responsive protein 27 ELISA Kit, Rat Heat shock 28 kDa protein
ELISA Kit, Rat Heat shock 25 kDa protein ELISA Kit, Rat estrogen-regulated 24 kDa protein ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat heat shock protein beta-1,HspB1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HspB1. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HspB1 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain HspB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HspB1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.