Rat Heparin cofactor II ELISA Kit


  • Model: ELA-E0284r
  • 20 Units in Stock
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Rat Heparin cofactor II ELISA Kit

96 Tests

Operating instruction



Alternative name

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Intended use

Rat Heparin cofactor II ELISA Kit allows for the in vitro quantitative determination of rat Heparin cofactor II concentrations in serum, Plasma, Urine,tissue homogenates and Cell culture supernates and Other biological fluids.


Test principle

The microtiter plate provided in Rat Heparin cofactor II ELISA Kit has been pre-coated with an antibody specific to Heparin cofactor II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Heparin cofactor II and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heparin cofactor II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heparin cofactor II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat Heparin cofactor II manual request

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