Rat Hepcidin, HEPC ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Hepcidin,Liver-expressed antimicrobial peptide 1,LEAP-1,Putative liver tumor regressor,PLTR, HAMP,hepcidin antimicrobial peptide;
hepcidin; HEPC; HFE2B; LEAP1; PLTR; liver-expressed antimicrobial peptide 1

Search name
Rat Hepcidin ELISA KIT,Rat Liver-expressed antimicrobial peptide 1 ELISA KIT,Rat LEAP-1 ELISA KIT,Rat Putative liver tumor
regressor ELISA KIT,Rat PLTR ELISA KIT,Rat HAMP ELISA KIT,Rat hepcidin antimicrobial peptide ELISA KIT,Rat hepcidin ELISA
KIT,Rat HEPC ELISA KIT,Rat HFE2B ELISA KIT,Rat LEAP1 ELISA KIT,Rat PLTR ELISA KIT,Rat liver-expressed antimicrobial
peptide 1 ELISA KIT,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Hepcidin concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Hepcidin, During the reaction, Hepcidin in the sample or standard competes with a fixed amount of
biotin-labeled Hepcidin for sites on a pre-coated Monoclonal antibody specific to Hepcidin. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Hepcidin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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