Rat Hyaluronidase- 1, HYAL1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HYAL1,HYAL,HYAL-1; LUCA1; Hyaluronidase-1, Hyaluronidase,MPS9; NAT6; luCa-1; lung carcinoma protein 1; plasma hyaluronidase; tumor suppressor LUCA-1; hyaluronoglucosaminidase 1, Hyaluronoglucosaminidase-1, LuCa-1

Search name

Rat HYAL1 ELISA KIT ,Rat HYAL ELISA KIT ,Rat HYAL-1 ELISA KIT ,Rat LUCA1 ELISA KIT ,Rat Hyaluronidase-1 ELISA KIT ,Rat  Hyaluronidase ELISA KIT ,Rat MPS9 ELISA KIT ,Rat NAT6 ELISA KIT ,Rat luCa-1 ELISA KIT ,Rat lung carcinoma protein 1 ELISA KIT ,Rat plasma hyaluronidase ELISA KIT ,Rat tumor suppressor LUCA-1 ELISA KIT ,Rat hyaluronoglucosaminidase 1 ELISA KIT ,Rat Hyaluronoglucosaminidase-1 ELISA KIT ,Rat LuCa-1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat hyaluronidase-1,Hyal1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyal-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hyal-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hyal-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hyal-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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