Rat Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain, ICTP ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain, ICTP, Carboxy-terminal telopepide type I collagen

Search name

Rat ICTP ELISA Kit, Rat Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain ELISA Kit, Rat Carboxy-terminal telopepide type I collagen ELISA Kit, ICTP ELISA Kit, Rat Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain ELISA Kit, Carboxy-terminal telopepide type I collagen ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain, ICTP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

AnchorTest principle

AnchorThe ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICTP, During the reaction, ICTP in the sample or standard competes with a fixed amount of biotin-labeled ICTP for sites on a pre-coated Monoclonal antibody specific to ICTP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICTP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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