Rat Interleukin 10, IL10 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interleukin-10,IL-10,Cytokine synthesis inhibitory factor , CSIF; GVHDS; IL10A; TGIF; T-cell growth inhibitory factor; cytokine synthesis
inhibitory factor; interleukin-10; Interleukin 10;

Search name
Rat Interleukin 10 ELISA Kit, Rat IL10 ELISA Kit, Rat Cytokine synthesis inhibitory factor ELISA Kit, Rat CSIF ELISA Kit, Rat
GVHDS ELISA Kit, Rat IL10A ELISA Kit, Rat TGIF ELISA Kit, Rat T-cell growth inhibitory factor ELISA Kit, Rat IL-10 ELISA Kit, Rat
Interleukin-10 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat interleukin-10,IL-10 concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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