Rat Interleukin 17, IL- 17 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Interleukin-17A,IL-17,IL-17A,Cytotoxic T-lymphocyte-associated antigen 8,CTLA-8,IL-17,IL17; CTLA8; IL-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17,cytotoxic T-lymphocyte-associated serine esterase 8,interleukin-17A; interleukin 17A

Search name

Rat Interleukin-17A ELISA KIT ,Rat IL-17 ELISA KIT ,Rat IL-17A ELISA KIT ,Rat Cytotoxic T-lymphocyte-associated antigen 8 ELISA KIT ,Rat CTLA-8 ELISA KIT ,Rat IL-17 ELISA KIT ,Rat IL17 ELISA KIT ,Rat CTLA8 ELISA KIT ,Rat IL-17A ELISA KIT ,Rat CTLA-8 ELISA KIT ,Rat cytotoxic T-lymphocyte-associated antigen 8 ELISA KIT ,Rat cytotoxic T-lymphocyte-associated protein 8 ELISA KIT ,Rat interleukin 17 ELISA KIT ,Rat cytotoxic T-lymphocyte-associated serine esterase 8 ELISA KIT ,Rat interleukin-17A ELISA KIT ,Rat interleukin 17A ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat interleukin-17a,IL-17 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-17. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-17 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.