Rat Interleukin- 2 receptor subunit Alpha, IL- 2RA/ CD25 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interleukin-2 receptor subunit alpha(IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA),CD25,IL-2-RA; IDDM10; IL2R;
TCGFR; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2-RA; TAC antigen; interleukin-2 receptor subunit alpha; p55; interleukin 2
receptor, alpha

Search name
Rat Interleukin-2 receptor subunit alpha ELISA Kit, Rat IL-2-RA ELISA Kit, Rat CD25 ELISA Kit, Rat IL-2 receptor subunit alpha
ELISA Kit, Rat IL-2R subunit alpha ELISA Kit, Rat IL-2-RA ELISA Kit, Rat IDDM10 ELISA Kit, Rat IL2R ELISA Kit, Rat TCGFR
ELISA Kit, Rat TAC antigen ELISA Kit, Rat p55 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat interleukin-2 receptor subunit alpha,IL-2-RA concentrations in
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-2-RA. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-2-RA and Avidin conjugated
to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain IL-2-RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-2-RA in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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