Rat Potassium voltage-gated channel subfamily B member 2, KCNB2 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Potassium voltage-gated channel subfamily B member 2,Voltage-gated potassium channel subunit Kv2.2, KCNB2,KV2.2,delayed rectifier potassium channel protein,potassium channel Kv2.2,potassium voltage-gated channel subfamily B member 2,voltage-gated potassium channel subunit Kv2.2,potassium voltage-gated channel, Shab-related subfamily member 2

Search name

Rat Potassium voltage-gated channel subfamily B member 2 ELISA KIT ,Rat Voltage-gated potassium channel subunit Kv2.2 ELISA KIT ,Rat KCNB2 ELISA KIT ,Rat KV2.2 ELISA KIT ,Rat delayed rectifier potassium channel protein ELISA KIT ,Rat potassium channel Kv2.2 ELISA KIT ,Rat potassium voltage-gated channel subfamily B member 2 ELISA KIT ,Rat voltage-gated potassium channel subunit Kv2.2 ELISA KIT ,Rat potassium voltage-gated channel ELISA KIT ,Rat  Shab-related subfamily member 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat potassium voltage-gated channel subfamily b member 2,KCNB2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to KCNB2, During the reaction, KCNB2 in the sample or standard competes with a fixed amount of biotin-labeled KCNB2 for sites on a pre-coated Monoclonal antibody specific to KCNB2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KCNB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.