Rat Keratin type I cytoskeletal 18,KRT18 ELISA Kit

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  • Model: ELA-E1231r
  • 20 Units in Stock
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Rat Keratin type I cytoskeletal 18, KRT18 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Keratin type I cytoskeletal 18,Keratin-18,K18,Cell proliferation-inducing gene 46 protein,Cytokeratin-18,CK-18, cytokeratin 18,CYK18; K18; CK-18; cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; cytokeratin-18; keratin type I cytoskeletal 18; keratin-18; keratin 18

 

Search name

Rat Keratin type I cytoskeletal 18 ELISA KIT ,Rat Keratin-18 ELISA KIT ,Rat K18 ELISA KIT ,Rat Cell proliferation-inducing gene 46 protein ELISA KIT ,Rat Cytokeratin-18 ELISA KIT ,Rat CK-18 ELISA KIT ,Rat  cytokeratin 18 ELISA KIT ,Rat CYK18 ELISA KIT ,Rat K18 ELISA KIT ,Rat CK-18 ELISA KIT ,Rat cell proliferation-inducing gene 46 protein ELISA KIT ,Rat cell proliferation-inducing protein 46 ELISA KIT ,Rat cytokeratin-18 ELISA KIT ,Rat keratin type I cytoskeletal 18 ELISA KIT ,Rat keratin-18 ELISA KIT ,Rat keratin 18 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Keratin, type I cytoskeletal 18 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin, type I cytoskeletal 18. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Keratin, type I cytoskeletal 18 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Keratin, type I cytoskeletal 18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Keratin, type I cytoskeletal 18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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