Rat LCN2 ELISA Kit

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  • Model: ELA-E1388r
  • 20 Units in Stock
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Rat neutrophil gelatinase- associated lipocalin/ lipocalin 2, LCN2/ NGAL ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

 

Alternative name

Rat LCN2 ELISA KIT ,Rat siderocalin LCN2 ELISA KIT ,Rat 24p3 ELISA KIT ,Rat MSFI ELISA KIT ,Rat NGAL ELISA KIT ,Rat p25 ELISA KIT ,Rat 25 kDa alpha-2-microglobulin-related subunit of MMP-9 ELISA KIT ,Rat lipocalin-2 ELISA KIT ,Rat migration-stimulating factor inhibitor ELISA KIT ,Rat oncogene 24p3 ELISA KIT ,Rat lipocalin 2 ELISA KIT ,Rat Neutrophil gelatinase-associated lipocalin ELISA KIT

 

Intended use

Rat LCN2 ELISA KIT allows for the in vitro quantitative determination of Rat neutrophil gelatinase- associated lipocalin/ lipocalin 2, LCN2/ NGAL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this Rat LCN2 ELISA KIT has been pre-coated with an antibody specific to LCN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LCN2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LCN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LCN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 15.6-1000pg/ml

Rat LCN2 ELISA KIT manual download

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