Rat Leptin receptor, LR/ Ob- R ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Leptin receptor(LEP-R),HuB219,OB receptor(OB-R), leptin receptor,CD295; LEP-R; LEPRD; OB-R; OBR; OB receptor; huB219
Search name
Rat Leptin receptor ELISA Kit, Rat LEP-R ELISA Kit, Rat Leptin receptor ELISA Kit, Rat LR ELISA Kit, Rat Ob-
R ELISA Kit, Rat HuB219 ELISA Kit, Rat OB receptor ELISA Kit, Rat CD295 ELISA Kit, Rat LEPRD ELISA Kit,
Rat OBR ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat leptin receptor,LEP-R concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP-R. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LEP-R and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain LEP-R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LEP-R in the samples is then determined by comparing
the O.D. of the samples to the standard curve.