Rat LTA4H ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  LTA4H

Protein Name  leukotriene A4 hydrolase

Alternative Name

Rat LTA4H ELISA KIT ,Rat leukotriene A4 hydrolase ELISA KIT ,Rat leukotriene A-4 hydrolase ELISA KIT ,Rat LTA-4 hydrolase ELISA KIT ,Rat testicular secretory protein Li 27 ELISA KIT

Intended use

Rat LTA4H ELISA KIT allows for the in vitro quantitative determination of LTA4H concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Rat LTA4H ELISA KIT has been pre-coated with an LTA4H antibody specific to LTA4H. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LTA4H and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LTA4H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTA4H in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20