Rat lipopolysaccharides, LPS ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Lipopolysaccharide, lipopolysaccharides,LPS, LOS, lipooligosaccharide,lipoglycans

Search name
Rat lipopolysaccharides ELISA Kit, Rat Lipopolysaccharide ELISA Kit, Rat LPS ELISA Kit, Rat LOS ELISA Kit, Rat
lipooligosaccharide ELISA Kit, Rat lipoglycans ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat lipopolysaccharide,Lipopolysaccharide concentrations in
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipopolysaccharide. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Lipopolysaccharide and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Lipopolysaccharide, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Lipopolysaccharide in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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