Rat Luteinizing Hormone ELISA Kit

96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Glycoprotein hormones alpha chain,Luteinizing hormone alpha chain(LSH-alpha),Follitropin alpha chain,Follicle-stimulating hormone
alpha chain(FSH-alpha),Chorionic gonadotrophin subunit alpha(CG-alpha),Thyrotropin alpha chain,Thyroid-stimulating hormone alpha
chain(TSH-alpha),Lutropin alpha chain,Anterior pituitary glycoprotein hormones common subunit alpha,Choriogonadotropin alpha chain,
Luteinizing Hormone, LH

Search Name
Rat Luteinizing Hormone ELISA Kit, Rat LH ELISA Kit,Rat Glycoprotein hormones alpha chain ELISA Kit, Rat CGA ELISA Kit, Rat FSHA ELISA Kit, Rat LHA ELISA Kit, Rat TSHA ELISA Kit, Rat LSH-alpha ELISA Kit, Rat FSH-alpha ELISA Kit, Rat CG-ALPHA ELISA Kit, Rat FSHA ELISA Kit, Rat GPHA1 ELISA Kit, Rat LHA ELISA Kit, Rat TSHA ELISA Kit, Rat FSH-alpha ELISA Kit, Rat TSH-alpha ELISA Kit,

Intended use
Rat Luteinizing Hormone ELISA Kit allows for the in vitro quantitative determination of rat glycoprotein hormones alpha chain,Glycoprotein hormones alpha chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in Rat Luteinizing Hormone ELISA Kit has been pre-coated with an antibody specific to Glycoprotein hormones alpha chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glycoprotein hormones alpha chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycoprotein hormones alpha chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycoprotein hormones alpha chain in the samples is then determined by comparing the O.D. of the samples to the
standard curve.

Rat Luteinizing Hormone ELISA Kit PDF Manual download