Rat Malondialdehyde ELISA Kit

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  • Model: ELA-E0597r
  • 20 Units in Stock
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Rat Malondialdehyde, MDA ELISA Kit
ELA-E0597r   96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Malondialdehyde,MDA

Search name
Rat Malondialdehyde ELISA Kit, Rat MDA ELISA Kit,Malondialdehyde ELISA Kit,MDA ELISA Kit,ELISA Kit, Malondialdehyde,MDA

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general malondialdehyde,Malondialdehyde concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Malondialdehyde, During the reaction, Malondialdehyde in the sample or standard competes with a fixed amount of biotin-labeled Malondialdehyde for sites on a pre-coated Monoclonal antibody specific to Malondialdehyde. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Malondialdehyde in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF manual download

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Reference

Article: Changes in nitric oxide, carbon monoxide, hydrogen sulfide and male reproductive hormones in response to chronic restraint stress in rats
Author:  AmiraMoustafa
Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt
Received 30 July 2020, Revised 1 September 2020, Accepted 22 October 2020, Available online 29 October 2020.
Free Radical Biology and Medicine
Available online 29 October 2020

DOI:doi.org/10.1016/j.freeradbiomed.2020.10.315

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