Rat Mast cell protease 6, Mcpt6 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Mcpt6,Tryptase beta-2,Mast cell protease 6,mMCP-6, Mcp6,TPSB2,TPS2; tryptaseB; tryptaseC; mast cell tryptase beta II; tryptase beta 2

Search name

Rat Mcpt6 ELISA KIT ,Rat Tryptase beta-2 ELISA KIT ,Rat Mast cell protease 6 ELISA KIT ,Rat mMCP-6 ELISA KIT ,Rat Mcp6 ELISA KIT ,Rat TPSB2 ELISA KIT ,Rat TPS2 ELISA KIT ,Rat tryptaseB ELISA KIT ,Rat tryptaseC ELISA KIT ,Rat mast cell tryptase beta II ELISA KIT ,Rat tryptase beta 2 ELISA KIT, Rat Tryptase II ELISA KIT

Intended use

Rat Mcpt6 ELISA KIT allows for the in vitro quantitative determination of rat Mast cell protease 6 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Rat Mcpt6 ELISA KIT has been pre-coated with an antibody specific to Mcpt6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mcpt6 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mcpt6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mcpt6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat Mcpt6 ELISA KIT Manual Request

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