Rat Matrix metalloproteinase 1, MMP 1 ELISA kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interstitial collagenase,Fibroblast collagenase,Matrix metalloproteinase-1(MMP-1), MMP-1,CLG; CLGN;

Search name
Rat Interstitial collagenase ELISA Kit, Rat Fibroblast collagenase 1 ELISA kit, Rat Matrix metalloproteinase 1 ELISA kit, Rat MMP
1 ELISA kit, Rat Matrix metalloproteinase-1 ELISA kit, Rat MMP-1 ELISA kit, Rat CLG ELISA kit, Rat CLGN ELISA kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat interstitial collagenase,Interstitial collagenase concentrations
in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interstitial collagenase. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Interstitial
collagenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Interstitial collagenase, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Interstitial
collagenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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