Rat myeloperoxidase ELISA Kit

Starting at: $756.00

  • Model: ELA-E0601r
  • 20 Units in Stock
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Rat Myeloperoxidase, MPO ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Myeloperoxidase(MPO),;

 

Search name

Rat MPO ELISA KIT ,Rat Myeloperoxidase ELISA KIT

 

Intended use

Rat myeloperoxidase ELISA Kit allows for the in vitro quantitative determination of rat myeloperoxidase,MPO concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Rat myeloperoxidase ELISA Kit has been pre-coated with an antibody specific to MPO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MPO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.78-50ng/ml

Rat myeloperoxidase ELISA Kit PDF Manual download

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