Rat Myoglobin ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat Myoglobin ELISA KIT ,Rat PVALB ELISA KIT ,Rat myoglobgin ELISA KIT ,Rat MYO ELISA KIT ,Rat MB ELISA KIT ,Rat MYO/ MB ELISA KIT
Intended use
Rat Myoglobin ELISA kit is for the in vitro quantitative determination of Rat Myoglobin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat Myoglobin ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myoglobin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myoglobin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Myoglobin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat Myoglobin ELISA kit Range 1.56-100ng/ml
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