Rat Myosin- binding protein C cardiac- type, MYBPC3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MYBPC3,CMD1MM; CMH4; FHC; LVNC10; MYBP-C; C-protein, cardiac muscle isoform; myosin-binding protein C, cardiac-type; myosin binding protein C cardiac
Search name
Rat MYBPC3 ELISA KIT ,Rat CMD1MM ELISA KIT ,Rat CMH4 ELISA KIT ,Rat FHC ELISA KIT ,Rat LVNC10 ELISA KIT ,Rat MYBP-C ELISA KIT ,Rat C-protein ELISA KIT ,Rat cardiac muscle isoform ELISA KIT ,Rat myosin-binding protein C ELISA KIT ,Rat cardiac-type ELISA KIT ,Rat myosin binding protein C cardiac ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Myosin- binding protein C cardiac- type, MYBPC3 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Myosin- binding protein C cardiac- type, MYBPC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Myosin- binding protein C cardiac- type, MYBPC3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Myosin- binding protein C cardiac- type, MYBPC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Myosin- binding protein C cardiac- type, MYBPC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
