Rat OAF (Out at first protein homolog) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Rat OAF ELISA KIT ,Rat OAF homolog ELISA KIT ,Rat NS5ATP13TP2 ELISA KIT ,Rat HCV NS5A-transactivated protein 13 target protein 2 ELISA KIT ,Rat out at first homolog ELISA KIT

Intended use

Rat OAF ELISA kit allows for the in vitro quantitative determination of Rat Out at first protein homolog concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Rat OAF ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to OAF, During the reaction, OAF in the sample or standard competes with a fixed amount of biotin-labeled OAF for sites on a pre-coated Monoclonal antibody specific to OAF. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product    Min.Order 5kits

Delivery time 4-6month

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