Rat paraoxonase 1, PON1 ELISA Kit


  • Model: ELA-E0243r
  • 20 Units in Stock
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Rat paraoxonase 1, PON1 ELISA Kit


96 Tests


Alternative name

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Intended use

Rat PON1 ELISA kit allows for the in vitro quantitative determination of Rat PON1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.


Test principle

The microtiter plate provided in Rat PON1 ELISA kit has been pre-coated with an antibody specific to PON1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PON1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PON1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.78-50ng/ml

Rat PON1 ELISA kit Manual download

Order Rat PON1 ELISA kit by email:sales@lifescience-market.com

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