Rat PAXIP1- associated protein 1, PA1 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PA1,PAGR1,C16orf53; GAS; PAXIP1-associated protein 1; PTIP-associated 1 protein; PTIP-associated protein 1; glutamate-rich coactivator associated with SRC1; glutamate-rich coactivator interacting with SRC1/NCOA1; PAXIP1 associated glutamate-rich protein 1

Search name

Rat PA1 ELISA KIT ,Rat PAGR1 ELISA KIT ,Rat C16orf53 ELISA KIT ,Rat GAS ELISA KIT ,Rat PAXIP1-associated protein 1 ELISA KIT ,Rat PTIP-associated 1 protein ELISA KIT ,Rat PTIP-associated protein 1 ELISA KIT ,Rat glutamate-rich coactivator associated with SRC1 ELISA KIT ,Rat glutamate-rich coactivator interacting with SRC1/NCOA1 ELISA KIT ,Rat PAXIP1 associated glutamate-rich protein 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of ratPAXIP1-associated protein 1concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toPAXIP1-associated protein 1, During the reaction,PAXIP1-associated protein 1in the sample or standard competes with a fixed amount of biotin-labeledPAXIP1-associated protein 1 for sites on a pre-coated Monoclonal antibody specific to PAXIP1-associated protein 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PAXIP1-associated protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.