Rat PIICP ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat PIICP ELISA KIT ,Rat Carboxyterminal propeptide of type II procollagen ELISA KIT ,Rat C- terminal propeptide of Collagen alpha- 1(II) chain ELISA KIT
Intended use
Rat PIICP ELISA kit is for the in vitro quantitative determination of Rat PIICP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat PIICP ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PIICP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PIICP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PIICP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat PIICP ELISA kit Range 15.6-1000pg/ml
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> PIICP Antidodies
Anti PIICP Antidody