Rat N-terminal propeptide of Collagen alpha-1(III) chain, PIIINP ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

N-terminal propeptide of Collagen alpha-1(III) chain,PIIINP, N- terminal procollagen III propeptide

Search name

Rat PIIINP ELISA Kit, Rat N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit, Rat N- terminal procollagen III propeptide ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat N-terminal propeptide of Collagen alpha-1(III) chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-terminal propeptide of Collagen alpha-1(III) chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for N-terminal propeptide of Collagen alpha-1(III) chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-terminal propeptide of Collagen alpha-1(III) chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N-terminal propeptide of Collagen alpha-1(III) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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