Rat Plasma alpha-L-fucosidase,Fuca2 ELISA KIT

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  • Model: ELI-30860r
  • 20 Units in Stock
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Rat Plasma alpha- L- fucosidase, FUCA2 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Fuca2,dJ20N2.5; alpha-L-fucosidase 2; alpha-L-fucoside fucohydrolase 2; plasma alpha-L-fucosidase; fucosidase, alpha-L- 2, plasma

 

Search name

Rat Fuca2 ELISA KIT ,Rat dJ20N2.5 ELISA KIT ,Rat alpha-L-fucosidase 2 ELISA KIT ,Rat alpha-L-fucoside fucohydrolase 2 ELISA KIT ,Rat plasma alpha-L-fucosidase ELISA KIT ,Rat fucosidase ELISA KIT ,Rat  alpha-L- 2 plasma ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Plasma alpha- L- fucosidase, FUCA2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FUCA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FUCA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FUCA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FUCA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

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