Rat platelet- associated Complement 1,PAC1/ DUSP2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DUSP2,PAC-1; PAC1; dual specificity protein phosphatase 2; dual specificity protein phosphatase PAC-1; dual-specificity phosphatase 2; serine/threonine specific protein phosphatase; dual specificity phosphatase 2
Search name
Rat DUSP2 ELISA KIT ,Rat PAC-1 ELISA KIT ,Rat PAC1 ELISA KIT ,Rat dual specificity protein phosphatase 2 ELISA KIT ,Rat dual specificity protein phosphatase PAC-1 ELISA KIT ,Rat dual-specificity phosphatase 2 ELISA KIT ,Rat serine/threonine specific protein phosphatase ELISA KIT ,Rat dual specificity phosphatase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Dual specificity protein phosphatase 2, DUSP2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Dual specificity protein phosphatase 2, DUSP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Dual specificity protein phosphatase 2, DUSP2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Dual specificity protein phosphatase 2, DUSP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Dual specificity protein phosphatase 2, DUSP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
