Rat Pleckstrin homology domain- containing family H member 3, PLEKHH3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PLEKHH3,PH domain-containing family H member 3; pleckstrin homology domain-containing family H member 3; pleckstrin homology domain containing, family H (with MyTH4 domain) member 3

Search name

Rat PLEKHH3 ELISA KIT ,Rat PH domain-containing family H member 3 ELISA KIT ,Rat pleckstrin homology domain-containing family H member 3 ELISA KIT ,Rat pleckstrin homology domain containing family H with MyTH4 domain member 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Pleckstrin homology domain- containing family H member 3, PLEKHH3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLEKHH3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PLEKHH3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PLEKHH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PLEKHH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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