Rat Protein G6b, G6B ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

G6b,protein G6b; NG31; immunoglobulin receptor; chromosome 6 open reading frame 25

Search name

Rat G6b ELISA KIT ,Rat protein G6b ELISA KIT ,Rat NG31 ELISA KIT ,Rat immunoglobulin receptor ELISA KIT ,Rat chromosome 6 open reading frame 25 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Protein G6b, G6B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein G6b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein G6b and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein G6b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein G6b in the samples is then determined by comparing the O.D. of the samples to the standard curve.