Rat P- Selectin, CD62P ELISA kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CD62P,P-selectin,CD62; GMP140; GRMP; LECAM3; PADGEM; PSEL; GMP-140,CD62 antigen-like family member P; GMP-140; granule membrane protein 140; CD62 antigen-like family member P,Granule membrane protein 140,granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; granule membrane protein 140kDa antigen CD62

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat P-selectin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-selectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for P-selectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain P-selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of P-selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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