Rat PTEN ELISA KIT

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  • Model: ELI-04890r
  • 20 Units in Stock
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Rat Phosphatidylinositol- 3,4,5- trisphosphate 3- phosphatase and dual- specificity protein phosphatase PTEN, PTEN ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

PTEN,phosphatidylinositol; 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; phosphatase and tensin-like protein; phosphatase and tensin homolog,3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

 

Search name

Rat PTEN ELISA KIT ,Rat phosphatidylinositol ELISA KIT ,Rat 10q23del ELISA KIT ,Rat BZS ELISA KIT ,Rat CWS1 ELISA KIT ,Rat DEC ELISA KIT ,Rat GLM2 ELISA KIT ,Rat MHAM ELISA KIT ,Rat MMAC1 ELISA KIT ,Rat PTEN1 ELISA KIT ,Rat TEP1 ELISA KIT ,Rat MMAC1 phosphatase and tensin homolog deleted on chromosome 10 ELISA KIT ,Rat mutated in multiple advanced cancers 1 ELISA KIT ,Rat phosphatase and tensin-like protein ELISA KIT ,Rat phosphatase and tensin homolog ELISA KIT,Rat 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA KIT, Rat phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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