Rat Putative phospholipase B-like 2, Plbd2 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Rat PLBD2 ELISA KIT ,Rat P76 ELISA KIT ,Rat 76 kDa protein ELISA KIT ,Rat LAMA-like protein 2 ELISA KIT ,Rat PLB homolog 2 ELISA KIT ,Rat lamina ancestor homolog 2 ELISA KIT ,Rat mannose-6-phosphate protein associated protein p76 ELISA KIT ,Rat phospholipase B domain-containing protein 2 ELISA KIT ,Rat phospholipase B-like 2 32 kDa form ELISA KIT ,Rat phospholipase B-like 2 45 kDa form ELISA KIT ,Rat phospholipase B domain containing 2 ELISA KIT ,Rat Putative phospholipase B-like 2 ELISA KIT ,Rat 66.3 kDa protein ELISA KIT

Intended use

Rat Plbd2 ELISA kit allows for the in vitro quantitative determination of Rat Putative phospholipase B-like 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Rat Plbd2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plbd2, During the reaction, Plbd2 in the sample or standard competes with a fixed amount of biotin-labeled Plbd2 for sites on a pre-coated Monoclonal antibody specific to Plbd2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Plbd2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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