Rat RAPGEF4 (Rap guanine nucleotide exchange factor 4) ELISA Kit (CUSTOM ELISA KIT)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Rat RAPGEF4 ELISA KIT ,Rat CAMP-GEFII ELISA KIT ,Rat CGEF2 ELISA KIT ,Rat EPAC ELISA KIT ,Rat EPAC 2 ELISA KIT ,Rat EPAC2 ELISA KIT ,Rat Nbla00496 ELISA KIT ,Rat RAP guanine-nucleotide-exchange factor (GEF) 4 ELISA KIT ,Rat Rap guanine nucleotide exchange factor (GEF) 4 ELISA KIT ,Rat cAMP-regulated guanine nucleotide exchange factor II ELISA KIT ,Rat exchange factor directly activated by cAMP 2 ELISA KIT ,Rat exchange protein directly activated by cAMP 2 ELISA KIT ,Rat Rap guanine nucleotide exchange factor 4 ELISA KIT

Intended use

Rat RAPGEF4 ELISA kit allows for the in vitro quantitative determination of Rat Rap guanine nucleotide exchange factor 4 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Rat RAPGEF4 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAPGEF4, During the reaction, RAPGEF4 in the sample or standard competes with a fixed amount of biotin-labeled RAPGEF4 for sites on a pre-coated Monoclonal antibody specific to RAPGEF4. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RAPGEF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized ELISA kit  Min.Order 5kits

Delivery time 4-6month

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