Rat reactive oxygen species, ROS ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ROS,ROS1, ROMO1, ROMO,ROS proto-oncogene 1 , receptor tyrosine kinase; v-ros avian UR2 sarcoma virus oncogene homolog 1; proto-oncogene c-Ros-1; c-ros oncogene 1 receptor tyrosine kinase; c-ros-1; MCF3; transmembrane tyrosine-specific protein kinase, Reactive oxygen species modulator 1.ROS modulator 1,Protein MGR2 homolog,Mitochondrial targeting GxxxG motif protein,MTGM,Protein MGR2 homolog,Glyrichin,
Search name
Rat ROS ELISA KIT ,Rat ROS1 ELISA KIT ,Rat ROMO1 ELISA KIT ,Rat ROMO ELISA KIT ,Rat ROS proto-oncogene 1 ELISA KIT ,Rat receptor tyrosine kinase ELISA KIT ,Rat v-ros avian UR2 sarcoma virus oncogene homolog 1 ELISA KIT ,Rat proto-oncogene c-Ros-1 ELISA KIT ,Rat c-ros oncogene 1 receptor tyrosine kinase ELISA KIT ,Rat c-ros-1 ELISA KIT ,Rat MCF3 ELISA KIT ,Rat transmembrane tyrosine-specific protein kinase ELISA KIT ,Rat Reactive oxygen species modulator 1.ROS modulator 1 ELISA KIT ,Rat Protein MGR2 homolog ELISA KIT ,Rat Mitochondrial targeting GxxxG motif protein ELISA KIT ,Rat MTGM ELISA KIT ,Rat Protein MGR2 homolog ELISA KIT ,Rat Glyrichin ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat reactive oxygen species modulator 1,ROS modulator 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ROS modulator 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ROS modulator 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ROS modulator 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ROS modulator 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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