Rat Serine racemase, SRR ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SRR,ILV1; ISO1; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; serine racemase

Search name

Rat SRR ELISA KIT ,Rat ILV1 ELISA KIT ,Rat ISO1 ELISA KIT ,Rat D-serine ammonia-lyase ELISA KIT ,Rat D-serine dehydratase ELISA KIT ,Rat L-serine ammonia-lyase ELISA KIT ,Rat L-serine dehydratase ELISA KIT ,Rat serine racemase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Serine racemase, SRR concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine racemase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serine racemase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serine racemase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serine racemase in the samples is then determined by comparing the O.D. of the samples to the standard curve.