Rat Serum albumin, SA ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Serum albumin, SA,albumin,serum albumin; PRO0883; PRO0903; PRO1341; 32 AA; albumin AA 34; cell growth inhibiting protein 42; growth-inhibiting protein 20,BSA

Search name

Rat Serum albumin ELISA KIT ,Rat  SA ELISA KIT ,Rat albumin ELISA KIT ,Rat serum albumin ELISA KIT ,Rat PRO0883 ELISA KIT ,Rat PRO0903 ELISA KIT ,Rat PRO1341 ELISA KIT ,Rat 32 AA ELISA KIT ,Rat albumin AA 34 ELISA KIT ,Rat cell growth inhibiting protein 42 ELISA KIT ,Rat growth-inhibiting protein 20 ELISA KIT ,Rat BSA ELISA KIT, Rat ALB ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat serum Serum albumin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum albumin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serum albumin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serum albumin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serum albumin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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