Rat Smoothened homolog, SMO ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SMO, Smoothened homolog ,FZD11; SMOH; frizzled family member 11; protein Gx; seven transmembrane helix receptor; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor; smoothened, frizzled class receptor ,
Search name
Rat SMO ELISA KIT ,Rat Smoothened homolog ELISA KIT ,Rat FZD11 ELISA KIT ,Rat SMOH ELISA KIT ,Rat frizzled family member 11 ELISA KIT ,Rat protein Gx ELISA KIT ,Rat seven transmembrane helix receptor ELISA KIT ,Rat smoothened ELISA KIT ,Rat frizzled family receptor ELISA KIT ,Rat smoothened ELISA KIT ,Rat seven transmembrane spanning receptor ELISA KIT ,Rat smoothened ELISA KIT ,Rat frizzled class receptor ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination ofratSMOconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SMO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SMO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SMO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SMO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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