Rat soluble Urokinase type Plasminogen Activator Receptor, suPAR

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  • Model: ELA-E14888r
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Rat soluble Urokinase type Plasminogen Activator Receptor, suPAR ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

sPLAUR,sMO,sCD87,soluble Urokinase plasminogen activator surface receptor,sU-PAR,suPAR, soluble Monocyte activation antigen Mo3,sURKR; soluble u-plasminogen activator receptor form 2; soluble urokinase plasmin

 

Search name

Rat suPAR ELISA KIT ,Rat sPLAUR ELISA KIT ,Rat sMO ELISA KIT ,Rat sCD87 ELISA KIT ,Rat soluble Urokinase plasminogen activator surface receptor ELISA KIT ,Rat sU-PAR ELISA KIT ,Rat  soluble Monocyte activation antigen Mo3 ELISA KIT ,Rat sURKR ELISA KIT ,Rat soluble u-plasminogen activator receptor form 2 ELISA KIT ,Rat soluble urokinase plasmin ELISA KIT

 

Intended use

Rat suPAR ELISA KIT allows for the in vitro quantitative determination of rat urokinase plasminogen activator surface receptor,uPAR concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Rat suPAR ELISA KIT has been pre-coated with an antibody specific to uPAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for uPAR and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain uPAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of uPAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 78-5000pg/ml

Rat suPAR ELISA KIT PDF Manual download

 

Citation for 

ELA-E14888r Rat soluble Urokinase type Plasminogen Activator Receptor, suPAR ELISA KIT

Inhibiting the urokinase‐type plasminogen activator receptor system recovers STZ‐induced diabetic nephropathy

Massimo Dal Monte  Maurizio Cammalleri  Valeria Pecci  Monica Carmosino  Giuseppe Procino Alessandro Pini  Mario De Rosa  Vincenzo Pavone  Maria Svelto  Paola Bagnoli
First published: 13 November 2018 
DOI: https://doi.org/10.1111/jcmm.14004

 

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