Rat Sorting nexin- 1, SNX1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SNX1,HsT17379; VPS5; sorting nexin 1A; sorting nexin-1; sorting nexin 1

Search name

Rat SNX1 ELISA KIT ,Rat HsT17379 ELISA KIT ,Rat VPS5 ELISA KIT ,Rat sorting nexin 1A ELISA KIT ,Rat sorting nexin-1 ELISA KIT ,Rat sorting nexin 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Sorting nexin- 1, SNX1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SNX1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SNX1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SNX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SNX1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.