Rat Sphingosine 1 Phosphate Receptor 5, S1PR5 ELISA kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Sphingosine 1-phosphate receptor 5(S1P receptor 5,S1P5),;
Sphingosine 1-phosphate receptor Edg-8(S1P receptor Edg-8),Endothelial differentiation G-protein-coupled receptor 8,Nerve growth factor-regulated G-protein-coupled receptor 1(NRG-1),;
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat sphingosine 1-phosphate receptor 5,S1P receptor 5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P receptor 5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S1P receptor 5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain S1P receptor 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S1P receptor 5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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