Rat Suppressor of cytokine signaling 3, Socs3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SOCS3,ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3; suppressor of cytokine signaling 3, SOCS-3

Search name

Rat SOCS3 ELISA KIT ,Rat ATOD4 ELISA KIT ,Rat CIS3 ELISA KIT ,Rat Cish3 ELISA KIT ,Rat SOCS-3 ELISA KIT ,Rat SSI-3 ELISA KIT ,Rat SSI3 ELISA KIT ,Rat STAT-induced STAT inhibitor 3 ELISA KIT ,Rat cytokine-inducible SH2 protein 3 ELISA KIT ,Rat suppressor of cytokine signaling 3 ELISA KIT ,Rat SOCS-3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat suppressor of cytokine signaling 3,SOCS-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOCS-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SOCS-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SOCS-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOCS-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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