Rat Thioredoxin mitochondrial, TXN2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Txn2,mitochondrial thioredoxin, thioredoxin,MT-TRX; MTRX; TRX2; thioredoxin, mitochondrial; thioredoxin-2; thioredoxin 2

Search name

Rat Txn2 ELISA KIT ,Rat mitochondrial thioredoxin ELISA KIT ,Rat  thioredoxin ELISA KIT ,Rat MT-TRX ELISA KIT ,Rat MTRX ELISA KIT ,Rat TRX2 ELISA KIT ,Rat thioredoxin mitochondrial ELISA KIT ,Rat thioredoxin-2 ELISA KIT ,Rat thioredoxin 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Thioredoxin mitochondrial, TXN2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TXN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TXN2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TXN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TXN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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