Rat Transmembrane glycoprotein NMB,Gpnmb ELISA KIT

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  • Model: ELI-31703r
  • 20 Units in Stock
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Rat Transmembrane glycoprotein NMB, GPNMB ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

GPNMB,HGFIN; NMB; glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; osteoactivin; transmembrane glycoprotein HGFIN; glycoprotein nmb, Transmembrane glycoprotein NMB,Osteoactivin,DC-HIL,Dendritic cell-associated transmembrane protein

 

Search name

Rat GPNMB ELISA KIT ,Rat HGFIN ELISA KIT ,Rat NMB ELISA KIT ,Rat glycoprotein (transmembrane) nmb ELISA KIT ,Rat glycoprotein nmb-like protein ELISA KIT ,Rat glycoprotein nonmetastatic melanoma protein B ELISA KIT ,Rat hematopoietic growth factor inducible neurokinin-1 ELISA KIT ,Rat osteoactivin ELISA KIT ,Rat transmembrane glycoprotein HGFIN ELISA KIT ,Rat glycoprotein nmb ELISA KIT ,Rat  Transmembrane glycoprotein NMB ELISA KIT ,Rat Osteoactivin ELISA KIT ,Rat DC-HIL ELISA KIT ,Rat Dendritic cell-associated transmembrane protein ELISA KIT

 

Intended use

Rat GPNMB ELISA KIT allows for the in vitro quantitative determination of rat transmembrane glycoprotein nmb,Transmembrane glycoprotein NMB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Rat GPNMB ELISA KIT has been pre-coated with an antibody specific to Transmembrane glycoprotein NMB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Transmembrane glycoprotein NMB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transmembrane glycoprotein NMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Transmembrane glycoprotein NMB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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