Rat Trhde ELISA KIT

Rat Thyrotropin- releasing hormone- degrading ectoenzyme, TRHDE ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

TRHDE,PAP-II; PGPEP2; TRH-DE; TRH-degrading ectoenzyme; TRH-specific aminopeptidase; pyroglutamyl aminopeptidase II; pyroglutamyl-peptidase II; thyroliberinase; thyrotropin-releasing hormone degrading ectoenzyme; thyrotropin-releasing hormone-degrading ectoenzyme; thyrotropin-releasing hormone degrading enzyme

 

Search name

Rat TRHDE ELISA KIT ,Rat PAP-II ELISA KIT ,Rat PGPEP2 ELISA KIT ,Rat TRH-DE ELISA KIT ,Rat TRH-degrading ectoenzyme ELISA KIT ,Rat TRH-specific aminopeptidase ELISA KIT ,Rat pyroglutamyl aminopeptidase II ELISA KIT ,Rat pyroglutamyl-peptidase II ELISA KIT ,Rat thyroliberinase ELISA KIT ,Rat thyrotropin-releasing hormone degrading ectoenzyme ELISA KIT ,Rat thyrotropin-releasing hormone-degrading ectoenzyme ELISA KIT ,Rat thyrotropin-releasing hormone degrading enzyme ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Thyrotropin- releasing hormone- degrading ectoenzyme, TRHDE beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Thyrotropin- releasing hormone- degrading ectoenzyme, TRHDE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Thyrotropin- releasing hormone- degrading ectoenzyme, TRHDE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Thyrotropin- releasing hormone- degrading ectoenzyme, TRHDE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Thyrotropin- releasing hormone- degrading ectoenzyme, TRHDE in the samples is then determined by comparing the O.D. of the samples to the standard curve.